The paper puts forward the model of mobile reading interactive behavior based on related research outputs of reading and communication and collaborative information behaviors, and makes an empirical study. The results show that most users communicate with others in mobile reading, readers who are 26-30 years old or with a bachelor's degree have the highest percentage of communication, readers with higher income have higher ratio of communication; instant communication tools and microblog are currently the most popular public platforms of mobile reading communication; Tencent, Sina, China Mobile, and Baidu's reading service platforms are the most popular special communities of mobile reading communication; most communication objects of mobile reading users are their friends, followed by other readers and authors; most communication content is review, followed by abstract, again is reading notes, labels, notation, etc; the most method of reading and communication is chatting and discussing, secondly is commenting, again is transmitting, writing blog, following, uploading, recommending; most extension behavior is querying related knowledge, secondly is accessing related website. Then, the paper proposes some targeted strategies for improving mobile reading service contents and methods based on the conclusion of the investigation analysis.