[Purpose/significance] "Center for Teaching Quality" and "Teacher Solutions 2030 Team" in the United States predict that teachers will become a mixed profession in the future, and the teaching regimen will provide a customized and meaningful learning program for students in a different manner. Subject librarians should actively explore and become a member of the teaching team. Based on the professional knowledge and skills of subject librarians, this paper proposes an embedded subject service model based on library resource organization.[Method/process] Using case analysis method, taking the University micro Lesson as an example, a framework of micro lesson teaching resource organization based on fragmentation knowledge unit is constructed, presenting in the superstar learning platform.[Result/conclusion] The research shows that subject information resources is the fundamental and foothold of subject service. Subject librarians, through efficient resource organization, discovery and presentation, realize the precision service oriented to the subject, thus becoming an organic part of the teaching and scientific research team in Colleges and universities. This research provides a new attempt for subject librarians to precisely embed the subject services.