[Purpose/significance] Investigating the current situation and predicament of subject service team construction in Chinese university libraries and putting forward specific suggestion from the perspective of the team have practical significance to improve the innovative service level of university libraries.[Method/process] Based on interviews with four university libraries in Shanghai and a questionnaire survey of fifty-two university libraries, this paper analyzes the current situation and predicament of the subject service teams construction of Chinese university libraries from six aspects,including the composition of subject service teams,the situation of full-time subject librarians,and the leaders of subject service team.[Result/conclusion] The existing staff structure of subject service team is reasonable,team leaders are getting younger,decision support,scientific research support and subject situation analysis have gradually become the focus of subject service work.However,there are some difficulties in the construction of subject service team,such as university libraries have different views on the importance of subject service,the challenge of subject librarian ability and knowledge reserve,and the lack of subject service team construction.